report science

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1. What was the gap in the field before this research? How do these scientists advance the field? What is the biological significance of this progress?

2. How do authors confirm that the developed blastoid resembles a mid/late blastocyst rather than post-implantation blastocyst? Give 2-3 different assays conducted (hint: answer based on Figure 2)

3. What are the differences in a) cell types b) gene expression and c) development stage between EPS- and ES-blastoid.

4. What is the significance of the Cdx2 gradient in the development of the blastoid/blastocyst? How does it compare to the gradient we have seen in the drosophila blastocyst?

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