BIO 120 Grossmont College Enzymes Lab
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Exercise 7 – ENZYMES for bio 120
Exercise 7: Enzymes
Adapted from Exercise 7: Enzymes (SIM 7/14/20)
I. PRELIMINARY OBSERVATIONS
A. INTRODUCTION: GENERAL INFORMATION ABOUT ENZYMES (READ)
B. THE ENZYME INVERTASE: A REPRESENTATIVE FOR TODAY’S STUDIES (READ)
Answer Questions 1 & 2
C. HOW TO TELL IF INVERTASE HAS DONE ITS JOB: BENEDICT’S TEST (READ)
D. BENEDICT’S TEST – PROCEDURE
Watch this video on Benedict’s test while reading the manual procedure.
E. USING BENEDICT’S TEST: HOW CAN IT BE USED TO DETECT FOR PRESENCE OF MONOSACCHARIDES (REDUCING SUGARS) LIKE GLUCOSE AND FRUCTOSE?
Write down the results from Benedict’s Test in Table 1.
Answer Questions 3 & 4
II. FACTORS AFFECTING ENZYME ACTIVITY
A. EXPERIMENT 1: EFFECT OF TEMPERATURE ON INVERTASE ACTIVITY
Answer Question 5
Watch this video showing the effect of Temperature on Enzyme Activity while reading the procedure
Effect of Temperature on Invertase
Fill in Table 2, and answer Questions 6 & 7
B. EXPERIMENT 2: EFFECT OF PH ON INVERTASE ACTIVITY
Answer Questions 8 – 10
Watch this video on Effect of pH on Enzyme Activity while reading the procedure.
Effects of pH on Invertase Activity
Fill in Table 3 and answer Questions 11 – 13.
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